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AGAROSES

Titan Media has different types of agarose, for eg. Agarose, (High EEO), Agarose, High EEO (0.23 - 0.26), Agarose, Low EEO, Agarose Spl. (Low EEO), Agarose (Low melting Temp. 65C) (Low Protoplast Culture), Agarose, Low EEO (0.5 - 0.13), Agarose, Medium EEO (0.16 - 0.19).

Agar is a heterogeneous mixture of two classes of polysaccharide: agaropectin and agarose. Both of the polysaccharide classes has same galactose based backbone and agaropectin is heavily modified with acidic side groups, such as sulphate and pyruvate. Agarose has a characteristic of forming a heat-reversible gel in dilute aqueous solution that is hydrophilic and macro porous, and does not contain charged groups or other adsorption centers. Agarose is being used in Gel - electrophoresis of DNA and Affinity chromatography. Agarose is used to determine the presence and distinguish the type of nucleic acids obtained after extraction and to analyze restriction digestion products.

The gel strength of each agarose is different. It is the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - in electrophoresis refers to the flow of water under the influence of an electric field due to immobilized charge groups on the matrix, primarily sulphate and carboxyl groups. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO, causing a net flow water through osmosis in that direction. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Products Code Products Name Technical Data
Packing.
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1227 AGAROSE
High EEO (0.23-0.26)
500.00 gm.
100.00 gm.
25.00 gm.
5.00 gm.
1267 AGAROSE
Extra High EEO (0.3)
500.00 gm.
100.00 gm.
25.00 gm.
5.00 gm.
1282 AGAROSE
Medium EEO (0.16-0.19)
500.00 gm.
100.00 gm.
25.00 gm.
5.00 gm.
1258 AGAROSE
Low EEO (0.05-0.13)
500.00 gm.
100.00 gm.
25.00 gm.
5.00 gm.
1268 AGAROSE, FP Spl.,
Low EEO Low Melting, DNA Grade
100.00 gm.
25.00 gm.
1270 AGAROSE, Spl.
Low EEO, For immunoelectrophoresis, Counterelectrophoresis
500.00 gm.
100.00 gm.
25.00 gm.
1271 AGAROSE, Spl.
Low EEO, With Very Low Viscosity
100.00 gm.
25.00 gm.
1272 AGAROSE
High Gel, With High Molecular Weight
100.00 gm.
25.00 gm.
1273 AGAROSE
Extra High Gel, Light Molecular Weight
100.00 gm.
25.00 gm.
1274 AGAROSE
Low Melting Temp. (65?C)
100.00 gm.
25.00 gm.
1275 AGAROSE
Medium Low Melting Temp. (63?C)
100.00 gm.
25.00 gm.
1276 AGAROSE
Extra Low Melting Temp. (60?C)
100.00 gm.
25.00 gm.
1278 AGAROSE
(Sieve) For Electro phoresis of DNA Fragments 71000 bp
100.00 gm.
25.00 gm.
1279 AGAROSE
For Molecular Screening, (Low Melting)
100.00 gm.
25.00 gm.
1280 AGAROSE
For Molecular Screening
100.00 gm.
25.00 gm.
1281 AGAROSE
Spl. for Molecular Screening
100.00 gm.
25.00 gm.
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