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Culture Medium

Culture media is special medium used in microbiological laboratories to grow different kinds of microorganisms. A growth or a culture medium is composed of different nutrients that are essential for a microbial growth. Microbiological growth medium - also called Culture Medium, or Nutrient Broth, solution freed of all microorganisms by sterilization (usually in an autoclave, where it undergoes heating under pressure for a specific time) and containing the substances required for the growth of microorganisms such as bacteria, protozoans, algae, and fungi. The medium gets solidified by the addition of agar. Since there are many types of microorganisms, each having unique properties and requiring specific nutrients for growth, the culture media are of many types based on what nutrients they contain and what function they play in the growth of microorganisms. This Culture medium or growth medium - a source of nutrients in which a microorganism is placed to permit its growth, cause it to produce substances, or observe its activity under defined conditions; also called culture medium or growth medium.

The medium is usually a solution of nutrients in water, or a similar solution solidified with gelatin or agar. The majority of the cell is made of carbon, oxygen, hydrogen, and nitrogen and phosphorous and these elements are necessary for forming membranes, proteins, nucleic acids and the other structures of the cell. These nutrients are required in large amounts, consisting of more than 1% of the cells dry weight and are termed macronutrients. Secondly, there are some elements that are required in lower concentrations are calcium, potassium, magnesium, sulfur, iron and manganese and these are called micronutrients. They make up between 0.1-1.0% of the cells dry weight micronutrients serve as less common parts of cellular structures or as necessary components for proteins. Although in smaller amounts, they too are absolutely required for the functioning of a living cell. Trace elements are compounds necessary in such small amounts that they are difficult to assign a requirement for. These are required in very small amounts (less than 0.1%) and their actual amount is difficult to measure. Growth factors examples include vitamins, amino acids and nucleotides. These nutrients are the chemical substances used by the organism for biosynthesis and energy generation. Bacteria must acquire all these elements from their environment for growth.

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